Scientists and students in the Soltis Lab are currently working with a variety of technologies to investigate mechanisms of speciation, evolutionary relationships and character evolution in flowering and land plants at all taxonomic levels. Current projects include the study of higher level phylogenetic relationships and character evolution in the angiosperms, floral evolution, the genetic and genomic consequences of polyploidy, conservation genetics of rare plants, phylogeography, and biogeography. Please explore our website for more information on our research.
Protocols at the Soltis Lab
Over the years, we’ve developed a number of lab protocols. Many of these are provided here and you are welcome to use and adapt them to your own needs. Several of these are older protocols that are no longer used in the lab, but users may still find them helpful.
- Soltis Lab CTAB DNA Extraction Protocol–still used with some modification
- DNA Extraction Protocol from Edwards 1991–quick and dirty DNA extraction
- Promega Wizard Kit DNA extraction protocol
- RNA Extraction Protocol for Basal Angiosperms
- Flow Cytometry Protocol for ploidy determination
- Histology Protocols
- Microsatellite Capture–old method with enrichment, cloning and Sanger sequencing
- PCR cleaning and sequencing
Code at the Soltis Lab
Many of the researchers in the Soltis lab have developed scripts and applications in the course of analyzing their data. We are in the process of making these more available and have established a github.com organization (did you know github.com offers free private repositories for education and researchers?) to share our code. The scripts range from quick and dirty scripts that are a few lines long to more complex and polished applications. Many students and visitors also have their own github repositories linked from here. We hope you find these resources helpful.
Our main lab repos: Soltis Lab on GitHub.com
Matt Gitzendanner’s repos: https://github.com/magitz
Paul Wolf, a visitor to the lab, has a bunch on github too: https://github.com/Wolflab